Agilent Series 1100 HPLC

System includes:

2 Solvent cabinets

2 Capillary pumps

1 Well-plate samplers

2 Autosampler thermostats

1 Thermostat column compartment

1 Diode array detector

1 Manual control module

Vacuum degasser cell missing

Capillary pump:

Adjustable column flow range: 0.01 - 20 μl/min

Recommended column flow range: 1 - 20 μl/min

Column flow precision: <0.7% RSD or 0.03% SD (typically 0.4% RSD or 0.02% SD), at 10 μl/min and 50 μl/min column flow (based on RT, default setting)

Optimal composite range: 1 to 99% or 5 μl/min per channel (primary flow), whichever is greater

Composite precision: <0.2% SD, at 10 μl/min (20 μl flow sensor), 50 μl/min (100 μl flow sensor) and 1 ml/min (normal mode) default setting

Delay volume: Typically 3 μl from the electronic flow control to the pump outlet for flows up to 20 μl / min.

Pressure range: 20 to 400 bar (5880 psi) system pressure

Compressibility compensation: User-selectable, based on mobile phase compressibility

Recommended pH range: 1.0 - 8.5, solvents with pH < 2.3 may not contain acids that corrode stainless steel. The upper pH range is limited by fused silica capillaries.

Plate sample:

Safety features: leak detection and safe leak handling, low voltages in service areas, error detection and display

Injection range: 0,01 - 8 μl in steps of 0,01 μl

Precision: Typically <0,5% RSD from peak areas of 5 - 40 μl, Typically <1% RSD from 1 - 5 μl, Typically <3% RSD from 0,2 - 1 μl

Sample Viscosity Range: 0,2 - 5 cp

Sample Capacity: 2 × platelets (MTP) + 10 × 2 ml vials, 100 x 2 ml in one tray, 40 x 2 ml in half a tray

Injection cycle time: Typically <30 s using the following standard conditions: Standard drawdown rate: 4 μl/min, Standard ejection rate: 10 μl/min, Injection volume: 0.1 μl

Carry-over: Typically <0.05% under the following conditions: Column: 150 x 0.5 mm Hypersil ODS, 3 μm

Mobile phase: Water/Acetonitrile = 85/15

Column flow rate: 13 μl/min

Injection volume: 1 μl caffeine (= 25 ng caffeine), 1 μl water to test transfer. Outside washing of needle before injection: 20 sec with water with rinsing port

Autosampler thermostat:

Temperature range: adjustable from 4°C to 40°C in 1°C increments

Temperature accuracy at ambient temperatures <25°C and humidity <50%: - 1 ° C to + 4 ° C at a set point of 4 ° C

Temperature accuracy at ambient temperatures > 25°C and/or humidity > 50%: - 1°C to +5°C at a setpoint of 4°C

Thermostat column compartment:

Temperature range: 10 degrees below ambient to 80 ° C, to 80 ° C: flow rates up to 5 ml / min

Temperature stability: ± 0,15 ° C

Temperature accuracy: ± 0.8 ° C (± 0.5 ° C on calibration)

Column capacity: Three 30 cm

Heating/cooling: 5 minutes from ambient to 40°C, 10 minutes from 40-20°C

Dead volume: 3 μl

Communication: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and close signals, LAN via other series module

Safety and maintenance: Comprehensive diagnostics, fault detection and display (via control module and Agilent ChemStation), leak detection, safe leakage handling, leakage signal for pump system shutdown. Low voltages in key maintenance areas.

Diode array detector:

Detection type: 1024-element photodiode array

Light source: Deuterium and tungsten lamps

Wavelength range: 190 - 950 nm

Short term noise (ASTM) Single and Multi-Wavelength: ± 1 × 10-5 AU at 254 and 750 nm

Drift: 2 × 10-3 AU / h at 254 nm

Linear absorption range: > 2 AU (upper limit)

Wavelength accuracy: ± 1 nm Self-calibration with deuterium lines, verification with holmium oxide filter

Wavelength bunching: 1 - 400 nm Programmable in 1 nm steps

Slit width: 1, 2, 4, 8, 16 nm Programmable slits

Diode width: <1 nm

Flow cells: 13 μl volume, 10 mm cell path length and 120 bar (1760 psi) pressure maximum

Control and data evaluation: Agilent ChemStation for LC

Analogue outputs: Recorder / integrator: 100 mV or 1 V, output range 0.001 - 2 AU, two outputs

Communication: Controller-area network (CAN), GPIB, RS-232C, APG Remote: ready, start, stop and close signals, LAN optional

Safety and maintenance: Comprehensive diagnostics, fault detection and display via control module and ChemStation), leak detection, safe leakage handling, leakage signal for pump system shutdown. Low voltages in key maintenance areas.

GLP features: Early Maintenance Feedback (EMF) for continuous tracking of instrument usage in terms of lamp burn time with user adjustable limits and feedback messages. Electronic records of maintenance and errors. Verification of wavelength accuracy with built-in holmium oxide filter.