Agilent 1290 Infinity HPLC-system

The Agilent 1290 Infinity LC system is designed to provide the greatest flexibility for performing analytical liquid chromatography using all types of current and emerging column technologies. The 1290 Infinity LC has the widest range of operating parameters, so it can replicate method settings transferred from any pre-existing analytical HPLC or UHPLC system available from any vendor. The Agilent 1290 Infinity LC system offers some radically new design concepts to achieve this goal, yet clearly demonstrates the pedigree of well-designed reliability that has made Agilent HPLC systems the most successful HPLC line.

System consists of:

Agilent 1290 Infinity Diode array detector

Agilent 1290 Infinity Thermostated Column Compartment

Agilent 1290 Infinity Binary Pump

Agilent 1290 Infinity Sampler

Agilent 1290 Infinity Thermostat

PC with Agilent Openlab Chemstation

Agilent 1290 Infinity Diode array detector:

Detection type: photodiode array with 1024 elements

Light source: Deuterium lamp

Wavelength range: 190 - 640 nm

Short-term noise: < ± 3 x 10-6 AU at 230 nm/4 nm, typically < ± 0.6 x 10-6 AU/cm at 230 nm/4 nm

Drift: < 0.5 x 10-3 AU/cm at 230 nm

Linear absorption range: > 2.0 AU (5%) at 265 nm

Wavelength accuracy: ± 1 nm (after recalibration with deuterium lines)

Beam wavelength: 2 - 400 nm, programmable in 1 nm steps

Slit width: 1, 2, 4, 8 nm

Diode width: ~0.5 nm

Flow cell: Max-Light cartridge cell (10 mm, V(σ) 1.0 µL)

Agilent 1290 Infinity Thermostated Column Compartment:

Temperature range: 10°C below ambient to 100°C (up to 80°C for flow rates up to 5 mL/min, up to 100°C for flow rates up to 2.5 mL/min)

Temperature stability: ± 0.05°C

Temperature accuracy: ± 0.8°C (± 0.5°C with calibration)

Column capacity: 3x 30 cm

Warm up/cool down time: 5 min from ambient temperature to 40°C, 10 min from 40 - 20°C

Dead volume: 1.6 µl low dispersion heat exchanger, 3 µl left heat exchanger, 6 µl right heat exchanger

Agilent 1290 Infinity Binary Pump:

Hydraulic System: Two double pistons in series pumps with patented servo-controlled variable stroke design, power

transmission by ballscrews, smooth motion control of pistons for active damping.

Adjustable flow range: 0.001-5 ml/min, in 0.001 ml/min

steps (performed in increments of 300 pl/step)

Flow precision: ≤0.07% RSD or 0.005 min SD, whichever is greater (0.2-5.0 ml/min)

Flow accuracy: ±1% or 10 µl/min, whichever is larger

Working pressure range: Working pressure range up to 120 MPa (1200 bar), up to 2 ml/min, 80 MPa (800 bar) at 5 ml/min.

Pressure pulsation: <1% amplitude or <0.5 MPa (5 bar), whichever is greater

Compensation of compressibility: automatic, predefined, based on selection of the mobile phase.

Gradient formation: binary mixing under high pressure.

Composition range: adjustable range 0 - 100% (recommended range: 1 - 99% or 5 µl/min per channel, whichever is greater)

Composition accuracy: <0.15% RSD, or 0.01 min SD, whichever is larger

Composition accuracy: ±0.35% absolute (5 - 95%, 0.2 - 5.0 ml/min)

Integrated degassing unit: 2 channels, internal volume per channel 1.5 mL

Agilent 1290 Infinity Sampler:

Injection range: 0.1 - 20 µL in 0.1 µL increments

Precision: <0.25% RSD from 5 - 20 µL, <0.5% RSD from 2 - 5 µL, <0.7% RSD from 1 - 2 µL

Sample viscosity range: 0.2 - 5 cp

Sample size: 2 x 54 vials

Injection cycle time: <21 s under standard conditions

Transfer rate: <0.004

Agilent 1290 Infinity Thermostat:

Temperature range: Adjustable from 4°C to 40°C in 1° increments

Temperature accuracy (ambient temperatures < 25°C and humidity < 50%): 3°C to 8°C at a setpoint of 4°C

Temperature accuracy (ambient temperatures <30°C and humidity <60%): 3°C to 9°C at a setpoint of 4°C