Ion Torrent Personal Genome Machine
The Ion Personal Genome Machine (PGM) system combines semiconductor sequencing technology with natural biochemistry to convert chemical information directly into digital data, democratize sequencing and make it accessible to your laboratory. This established system takes advantage of the exponential improvements in the semiconductor industry (known as Moore's Law) to provide scalability and flexibility for a variety of applications. The Ion PGM system uses natural sequencing chemistry, eliminating the need for expensive optical objects and reducing complex chemical processes to measure natural DNA expansion. The system uses direct, real-time detection sequencing and provides sequencing results typically within 3-7 hours.
CPU: Dual 6-core 2.66 GHz CPUs
Capacity: 1 chip per run
Chip compatibility: Ion 314 Chip v2 BC, Ion 316 Chip v2 BC, Ion 318 Chip v2 BC
Current limit: 6.7 A (max)
Data output: BAM files, FASTQ files, SFF files, VCF files
Display interface: Touch screen user interface
External computer: Torrent server
High Throughput Compatibility: High Throughput Compatible, Multiplexing
Instrument memory: 48 GB RAM, Eight 2 TB hard drives in RAID 5
Operating System: Ubuntu 10.04
Output: Ion 314 Chip v2: up to 100 Mb, Ion 316 Chip v2: up to 1 GB, Ion 318 Chip v2: up to 2 GB
Read length: 35 - 400 bases (average: 200 bases)
Reads / Run: Ion 314 Chip v2: 400-500 thousand, Ion 316 Chip v2: 2-3 million, Ion 318 Chip v2: 4-5.5 million
Run Time: Ion 314 Chip v2: 2.3 to 3.7 hours, Ion 316 Chip v2: 3.0 to 4.9 hours, Ion 318 Chip v2: 4.4 to 7.3 hours
Sequencing Scale: 3 Mb to 2 Gb
Technique: Post-Light Ion Semiconductor Sequencing
Throughput: up to 2 GB per run
Voltage limit: 100 V (min) to 240 V (max)
Dimensions (H x W x D): 530 x 240 x 200 mm
Weight: 30 kg
The Ion OneTouch 2 system performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any work surface.
The Ion OneTouch 2 system integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM system and up to 200 bases on the Ion Proton system. The need for gas is eliminated by the use of a peristaltic pump.
Chip compatibility: Ion 314 Chip, Ion 316 Chip, Ion 318 Chip, Ion PI Chip
High Throughput Compatibility: Automated Protocols, High Throughput Compatible, Multiplexing
Library Type: Fragment library, Mate paired library, Targeted sequence library, cDNA library
Multiplex Capability: up to 384 barcoded libraries
Running time: Hands-on time: minutes, Ion PGM system: total 5 hours for template preparation