The Thermo Scientific PikoReal 24-cycler is ideal for starting real-time PCR with low sample throughput (24-well format). The PikoReal software provides simple and intuitive operation of the cycler and allows easy online control from your computer. The cycler has 5 detection channels, which detect hydrolysis (5'Nuclease / TaqMan) probes as single-color or multiplex samples.

Thermal block

Block size: 24 wells

Sample volume: 10 - 50 μl

Consumables: low profile strips and PCR tubes

Max heating rate: > 5°C/sec

Max cooling rate: 4.5°C/sec

Temperature range: 4-99.9°C

Temperature accuracy: ±0.2°C

Temperature uniformity: ±0.3°C at 95°C, ±0.15°C at 60°C, ±0.2°C at 72°C

Heated lid

Temperature range: 50-110°C

Control: Automatic temperature and pressure setting

Optics

Excitation: 5 LEDs

Excitation range: 475-640 nm

Detection: CCD

Detection channels

Channel 1 - Excitation: 475-500nm, Emission: 520-550nm, Pre-calibrated dyes: FAM

Channel 2 - Excitation: 515-535nm, Emission: 557-590nm, Pre-calibrated dyes: HEX, Yakima Yellow

Channel 3 - Excitation: 570-590nm, Emission: 615-650nm, Pre-calibrated dyes: ROX, Texas Red

Channel 4 - Excitation: 600-640nm, Emission: 666-740nm, Pre-calibrated dyes: Cy 5

Channel 5 - Excitation: 475-500nm, Emission: 520-590nm, Pre-calibrated dyes: SYBR Green

Multiplex: up to 4 targets

Dynamic Range: 10 logs

Sensitivity: 1 copy

Analysis modes

Absolute quantification; Relative quantification; Melt curve

analysis; Allelic discrimination; High resolution melt (HRM)

Stand-alone or PC control:

The PikoReal qPCR system is controlled by PikoReal software, which allows remote monitoring of any qPCR experiment online from your own computer. Alternatively, you can save the qPCR protocol on a USB stick and load to the PikoReal instrument for stand-alone use.

PikoReal software include modules for absolute and relative quantification, melting curve analysis, allelic discrimination and High Resolution Melting (HRM). Advanced algorithms provide effortless data normalization without the need for an external reference dye.