Agilent 1260 Infinity Preparative HPLC System

Agilent 1260 Infinity system consisting of:

2x Agilent 1260 Prep pump

1x Agilent 1260 FC-PS Preparative Scale Fraction Collector

1x Agilent 1260 Multiwavelength Detector

Solvent tray

Manual injector with 10 ml sample loop

PC and software

Agilent 1260 Prep Pump:

The Agilent 1200 series preparative pump delivers a stable and reproducible flow at both low and high flow rates. By adding a second pump, a high-pressure gradient system can be easily assembled.

Features:

Flow rates up to 100 ml/min at 400 bar without changing pump heads

Precise formation of binary gradients under high pressure with low delay volume for preparative and analytical applications with flow rates as low as 0.5 ml/min

Extended seal life through automated seal washing

Setting upper and lower pressure limits switches the pump off, e.g. if the column is clogged or if leakage occurs

Convenient system configuration by automated solenoid bleed valve

Specifications:

Flow precision: <0.3% RSD in range of 1 ml/min to 100 ml/min

Hydraulic system: double pistons in parallel

Flow range: set points from 0.001 to 100 ml/min, in increments of 0.001 ml/min Recommended: 5 - 100 ml/min

Pressure: Operating range 2 - 40 MPa (20 - 400 bar, 290 - 5880 psi) system pressure

Gradient formation: High pressure with two combined pumps

Delay volume: 700 µL

pH range: 1.0 - 12.5 recommended

Agilent 1260 FC-PS Preparative Scale Fraction Collector

Specifications:

Ttrigger modes: time intervals, peak (threshold, up/downslope),

Timing (combination of time intervals and peak).

Operating modes: Discrete fractions - standard mode for all vessels. Flow is diverted to waste as it moves from one vessel position to the next vessel position.

Fraction capacities: Full tray with 126 test tubes (16 mm OD, max. height 100 mm, ~12 ml/tube)

Installed trays are automatically detected and identified.

Only for vials, test tubes and well plates that have been uncapped!

Maximum tube volume: approx. 45 ml

Maximum flow rate: 100 ml/min (depending on viscosity and generated counterpressure, max. 6 bar at the diverter valve)

Delay volumes [μl]: Inlet from fraction collector to diverter valve: ~500 (typical, depends on hose length), diverter valve: ~15, diverter valve to needle: ~110, needle: ~5

Delay calibration sensor: Single wavelength absorption detector operating at 654 nm, consisting of a LED and a photodiode

Switching valve: 3/2 Switching valve with low internal volume (15 μl), switching time < 100 ms, maximum working pressure 6 bar

Agilent 1260 Multiple Wavelength Detector

Specifications:

Detection type: photodiode array with 1024 elements

Light source: Deuterium and tungsten lamps

Data rate: up to 20 Hz

Wavelength range: 190 - 950 nm

Short term noise (ASTM) Single and multiple wavelengths: < ± 0.7-10AU at 254 and 750 nm

Drift: < 0.9-10AU/h at 254 nm

Linear absorption range: > 2 AU (5%) at 265 nm

Wavelength accuracy: ± 1 nm

Beam wavelength: 1 - 400 nm

Slit width: 1, 2, 4, 8, 16 nm

Diode width: < 1 nm

Flow cell: 13 µl volume, 10 mm cell length

Time programmable: Wavelength, polarity, peak width, lamp bandwidth, autobalance, wavelength range, threshold, spectral storage mode